The second VP test was probably contaminated and was not a reliable test result. These tubes were incubated at 37 degrees for two days.
Lab Manual for General Microbiology. Like other bacteria in the Bacillaceae family, B.
After isolating the second bacteria, a Gram stain was performed, hoping to see a purple color, indicating a gram positive.
A positive catalase test removed E. Tables 1 and 2 lists the tests, purposes, reagents used and the results of each test.
However, the researcher felt it was important to perform a battery of tests in order to confidently conclude that the Gram positive unknown was indeed B. The streak plate was then incubated at 37 degrees Celsius for 48 hours. Tortora, Gerard J. This bacterium is actively motile and swarming behavior.
Returning to the original unknown stocka quadrant streak plate was done with a sterilized inoculating look on a mannitol salt agar, which inhibits the growth of gram negative bacteria. It is predicted that it spends most of its time inactive and in spore form. However, because it was gram-positive Rods, this can eliminate the last 3, leaving Bacillus cereus and Bacillus subtilis.
Since the original Unknown test tube was not allowing the gram negative to grow, an Alternative 4 tube was assigned as well.