How to write an unknown lab report in microbiology general

The second VP test was probably contaminated and was not a reliable test result. These tubes were incubated at 37 degrees for two days.

Lab Manual for General Microbiology. Like other bacteria in the Bacillaceae family, B.

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After isolating the second bacteria, a Gram stain was performed, hoping to see a purple color, indicating a gram positive.

A positive catalase test removed E. Tables 1 and 2 lists the tests, purposes, reagents used and the results of each test.

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However, the researcher felt it was important to perform a battery of tests in order to confidently conclude that the Gram positive unknown was indeed B. The streak plate was then incubated at 37 degrees Celsius for 48 hours. Tortora, Gerard J. This bacterium is actively motile and swarming behavior.

Returning to the original unknown stocka quadrant streak plate was done with a sterilized inoculating look on a mannitol salt agar, which inhibits the growth of gram negative bacteria. It is predicted that it spends most of its time inactive and in spore form. However, because it was gram-positive Rods, this can eliminate the last 3, leaving Bacillus cereus and Bacillus subtilis.

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A negative nitrate test ruled out E. Firstly, the Gram positive unknown was rod shape. Proteus vulgaris is found in human intestinal tract, soil, water and plants. There were multiple procedures and test done in order to gain all the information needed to determine which bacteria was given. This completed the search for the Gram positive rod: B. Firstly, each of the tests in the series ruled out a possible candidate until P. Some other commercial applications of B. McDonald, Virginia et al. However, the researcher felt it was important to perform a battery of tests in order to confidently conclude that the Gram positive unknown was indeed B. For example, a nutrient agar stock plate for B. Next, the MacConkey and EMB tests were performed to detect if the bacterium was an enteric and fermenter of lactose. Accessed April 27, However, because it was gram-positive Rods, this can eliminate the last 3, leaving Bacillus cereus and Bacillus subtilis. Glycerol and Maltose test were then performed in hopes of confirming this bacterium. A second isolation was completed and when observed it confirmed that only two bacterium grew.

Since the original Unknown test tube was not allowing the gram negative to grow, an Alternative 4 tube was assigned as well.

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Unknown Microbiology Lab Report Essay